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Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins BJM
Mesquita,Adriano Queiroz de; Rezende,Cintia Silva Minafra e; Mesquita,Albenones José de; Jardim,Eurione Antonio Garcia da Veiga; Kipnis,Ana Paula Junqueira.
The identification of dairy cows with greater or lower potential to develop mastits has been pursued for many years among different segments of the milk industry, including governmental organizations. Genomic studies have suggested that Single Nucleotide Polymorphisms (SNPs) within the pattern recognition receptors (PRR) could lead to different responses to pathogens, and consequently result in mastitis resistance or susceptibility. To investigate whether toll like receptor 4 (TLR4) gene is associated with subclinical mastitis in Holstein cows from a property in the state of Goiás, Brazil, TaqMan allelic discrimination and somatic cell count were performed. One hundred and fifty milk samples were analyzed for SCC and centesimal composition. Twenty percent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: TLR-4; Single nucleotide polymorphism; Mastitis; Milk quality.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200034
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PCR and ELISA (VIDAS ECO O157®) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO BJM
Carvalho,Rosangela Nunes; Oliveira,Antonio Nonato de; Mesquita,Albenones José de; Rezende,Cíntia Silva Minafra e; Mesquita,Adriano Queiroz de; Romero,Rolando Alfredo Mazzoni.
Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157®, bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Minas Frescal cheese; STEC/VTEC; PCR; VIDAS ECO O157®.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100002
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